In situ imidazole activation of ribonucleotides for abiotic RNA oligomerization reactions.
Identifieur interne : 001750 ( Main/Exploration ); précédent : 001749; suivant : 001751In situ imidazole activation of ribonucleotides for abiotic RNA oligomerization reactions.
Auteurs : Bradley T. Burcar [États-Unis] ; Mohsin Jawed ; Hari Shah ; Linda B. McgownSource :
- Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life [ 1573-0875 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Bentonite, Imidazoles, RNA, Ribonucleotides.
- Catalysis, Evolution, Chemical.
Abstract
The hypothesis that RNA played a significant role in the origin of life requires effective and efficient abiotic pathways to produce RNA oligomers. The most successful abiotic oligomerization reactions to date have utilized high-energy, modified, or pre-activated ribonucleotides to generate strands of RNA up to 50-mers in length. In spite of their success, these modifications and pre-activation reactions significantly alter the ribonucleotides in ways that are highly unlikely to have occurred on a prebiotic Earth. This research seeks to address this problem by exploring an aqueous based method for activating the canonical ribonucleotides in situ using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and imidazole. The reactions were run with and without a montmorillonite clay catalyst and compared to reactions that used ribonucleotides that were pre-activated with imidazole. The effects of pH and ribonucleotide concentration were also investigated. The results demonstrate the ability of in situ activation of ribonucleotides to generate linear RNA oligomers in solution, providing an alternative route to produce RNA for use in prebiotic Earth scenarios.
DOI: 10.1007/s11084-015-9412-y
PubMed: 25716919
Affiliations:
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The most successful abiotic oligomerization reactions to date have utilized high-energy, modified, or pre-activated ribonucleotides to generate strands of RNA up to 50-mers in length. In spite of their success, these modifications and pre-activation reactions significantly alter the ribonucleotides in ways that are highly unlikely to have occurred on a prebiotic Earth. This research seeks to address this problem by exploring an aqueous based method for activating the canonical ribonucleotides in situ using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and imidazole. The reactions were run with and without a montmorillonite clay catalyst and compared to reactions that used ribonucleotides that were pre-activated with imidazole. The effects of pH and ribonucleotide concentration were also investigated. The results demonstrate the ability of in situ activation of ribonucleotides to generate linear RNA oligomers in solution, providing an alternative route to produce RNA for use in prebiotic Earth scenarios. </div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Jawed, Mohsin" sort="Jawed, Mohsin" uniqKey="Jawed M" first="Mohsin" last="Jawed">Mohsin Jawed</name><name sortKey="Mcgown, Linda B" sort="Mcgown, Linda B" uniqKey="Mcgown L" first="Linda B" last="Mcgown">Linda B. Mcgown</name><name sortKey="Shah, Hari" sort="Shah, Hari" uniqKey="Shah H" first="Hari" last="Shah">Hari Shah</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Burcar, Bradley T" sort="Burcar, Bradley T" uniqKey="Burcar B" first="Bradley T" last="Burcar">Bradley T. Burcar</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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